Ph.D. Program in Biomedical Sciences and Oncology

Contatti

Supervisore

Curriculum vitae

Tesi di dottorato

CIT-K is  a large multidomain protein, containing a very conserved amino-terminal serine/threonine kinase domain and is one of the many effectors of Rho small GTPases, cytoskeletal regulators that play pleiotropic roles in cells. CIT-K knockdown results in a cytokinesis phenotype in Hela cells. In particular HeLa cells fail the cytokinesis during a very late stage, commonly referred to as abscission. Conversely the analysis of the phenotype of CIT-K knockout mice led to the discovery that CIT-K is required for cytokinesis only in specific cell types in particular GNP  and testis germ cells. We demonstrated that microtubules stability are important for CIT-K knockdown phenotypes and in particular that  Citron could have stabilizing effects on tubulin during abscission. We prove also that a particular tubulin isoform is important for citokynesis  (Tubulin BetaIII). In particular Tubulin betaIII downregulation and overespression determinate a binucleate phenotypes; therefore  Citron and this tubulin double knockdown combine to generate a lot of  binucleate cell in Hela, indicating that both are important for cytokinesis. We must understand if Cit-K  regulate tubulin betaIII dynamics and how this regulation occur. Since tubulin betaIII is expressed in neuronal cell, we translate the evidence obtained in hela in a neuronal cellular system

Attività di ricerca

 corso di aggiornamento “cellule Staminali in modelli sperimentali preclinici e clinici”

 36^th  FEBS Congress "Biochemistry for Tomorrow's Medicine"Torino

Abstract e Poster "Microtubule-dependent cytokinesis control by Citron kinase" presentato Al FEBS Congress "Biochemistry for Tomorrow's Medicine.

corso teorico " Cellular senescence, in anging, stem cell biology, tumor suppression and therapy"

 WORKSHOP “ZEISS on Your CAMPUS” svolto Presso l’ UNIVERSITÀ DEGLI STUDI di Torino MOLECULAR BIOTECHNOLOGY CENTER

Partecipazione al Keystone Simposia “Molecular, Cellular, Physiological, and Pathogenic Responses to Hypoxia “,  Fairmont Hotel Vancouver  •  Vancouver, British Columbia

convegno “Molecular Determinants of cell Signalling”, organizzato dalla società di biofisica e biologia molecolare, svoltosi presso il centro di biotecnologie molecolari dell’università’ di Torino

 ABCD “Stem cell, development, regeneration” Torino, 6-7  Maggio 2012

 “Cancer day”, Torino, 7  Giugno 2012